Fimbrin, a new microfilament-associated protein present in microvilli and other cell surface structures
نویسندگان
چکیده
A 68,000 mol wt polypeptide has been identified as one of the few major proteins in the microfilament bundles of the microvilli present on intestinal epithelial cells. Antibodies against the purified protein have been used in indirect immunofluorescence microscopy on several cultured cells. The protein have been used in indirect immunofluorescence microscopy on several cultured cells. The protein is found particularly prominent in membrane ruffles, microspikes, and microvilli.
منابع مشابه
Microinjection of villin into cultured cells induces rapid and long- lasting changes in cell morphology but does not inhibit cytokinesis, cell motility, or membrane ruffling
Villin, a Ca2(+)-regulated F-actin bundling, severing, capping, and nucleating protein, is a major component of the core of microvilli of the intestinal brush border. Its actin binding properties, tissue specificity, and expression during cell differentiation suggest that it might be involved in the organization of the microfilaments in intestinal epithelial cells to form a brush border. Recent...
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Fimbrin is a cytoskeletal protein associated with microfilaments in microvilli, microspikes, stereocilia, membrane ruffles, and cell--substratum attachment sites. Fimbrin purified from intestinal epithelial cell brush borders was found to be a monomeric protein of molecular weight 68,000. In a sedimentation assay, fimbrin bound to F-actin in a salt-dependent manner, with binding being optimal i...
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ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 86 شماره
صفحات -
تاریخ انتشار 1980